A diet based on cured acorn ham with oleic acid content promotes anti-inflammatory gut microbiota shifts and prevents ulcerative colitis in an animal model
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A diet based on cured acorn-fed ham with oleic acid content promotes anti-inflammatory gut microbiota and prevents ulcerative colitis in an animal model.
Abstract
<h4>Background</h4>Diets based on meat products are not recommended in the case of ulcerative colitis (UC). The objective here is to test if some traditional cured meat products, as acorn-fed ham (high levels of oleic acid), may be useful for controlling inflammatory diseases as UC in animal models, which could represent a new dietary complementary intervention in the prevention of this inflammatory disease in humans.<h4>Methods</h4>Two rat cohorts have been used: conventional vegetable rat feed and acorn-fed ham. UC was induced with DSS in drinking water ad libitum for 1 week. Short-chain fatty acids (SCFAs) and 16S rRNA metagenomics from bacterial populations were analyzed in cecum samples. Colon samples were analyzed for histological parameters.<h4>Results</h4>Acorn-fed ham diet induced changes in gut microbiota composition, with pronounced enrichments in anti-inflammatory bacterial genera (Alistipes, Blautia, Dorea, Parabacteroides). The animals with this diet showed a strong reduction in most parameters associated to ulcerative colitis: disease activity index, macroscopic score of colitis, epitelium alteration in colon mucosa, inflammatory cell density in colon, myeloperoxidase titers in colon, proinflammatory cytokines (IL-17, IFN-γ). Also, acorn-fed ham diet animals showed increased total antioxidant activity an oleic acid levels in plasma, as well as higher short-chain fatty acid concentrations in cecum (isobutyric, isovaleric and valeric).<h4>Conclusions</h4>In the acorn-fed ham cohort, as a result of the dietary intake of oleic acid and low intake of omega-6 fatty acids, a strong preventive effect against UC symptoms was observed.
doi:10.1186/s12944-020-01205-x ↗ PMID 32093685 ↗ PMC7041278 ↗
Study facts
- Organism
- —
- Platform
- Ion Torrent PGM
- Age group
- —
- Disease groups
- —
- Anatomical sites
- —
Data availability
Specific data assets have not been resolved from the source yet — see the source repository below for the full file listing.
Strengths & limitations for reuse
Strengths
- Raw reads are advertised
- Taxonomic tables are advertised
- Sample counts are documented
Limitations
- Not documented: feature/otu tables are advertised
- Not documented: participant counts are documented
Extraction evidence & provenance
Each extracted field is shown with the source excerpt and location used to resolve it.
Assay
| Field | Value | Evidence |
|---|---|---|
assay.platform |
Ion Torrent PGM from source |
ENA instrument_model=Ion Torrent PGM Section |
Cohort
| Field | Value | Evidence |
|---|---|---|
cohort.disease_activity_metadata_available |
True |
The rats were monitored weekly for food and drinking water intake, weight loss and stool consistency/rectal bleeding using a modified protocol ... on UC disease activity index (DAI). Section |
cohort.treatment_exposure_documented |
True |
UC was induced in 8 rats from each cohort. Induction was carried out using autoclaved drinking water containing 3% DSS ... for 7 days Section |
cohort.treatment_response_metadata_available |
True |
Finally, disease activity index (DAI) was measured in all animals. DAI values were higher in the feed cohort ... than in acorn-fed ham cohort Section |
Data_Assets
| Field | Value | Evidence |
|---|---|---|
data_assets.open_access |
True |
"isOpenAccess": "Y" Section |
data_assets.pipeline_or_tool_versions |
True |
Taxonomic adscription up to species level was performed using the QIIME 2 (v.2017.6.0) open-source bioinformatics pipeline. Section |
data_assets.raw_reads |
True |
All raw metagenomics data have been deposited at NCBI SRA database (submission PRJNA524796). Section |
data_assets.taxonomic_table |
True |
This excel table includes the percentages for each taxonomic level and was used for comparing frequencies between rat individuals and cohorts. Section |
Specimens
| Field | Value | Evidence |
|---|---|---|
specimens.body_site |
cecum |
the cecum (frozen at − 20 °C) Section |
specimens.inflamed_status_available |
True |
UC was induced in 8 rats from each cohort... Two rats from each cohort were left free of DSS as absolute controls (no UC). Section |
specimens.number_of_samples |
24 from source |
ENA sample_count=24 Section |
specimens.sample_type |
stool |
gDNA was extracted from 200 mg of frozen (− 80 °C) cecum feces Section |